Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo
نویسندگان
چکیده
Photoacoustic imaging (PAI) is a hybrid non-invasive technique used to merge high optical contrast and acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include depth, resolution, label-free imaging. As representative implementation of PAI, photoacoustic microscopy (PAM) preclinical clinical studies for micron-scale spatial capability with absorption contrast. Several handheld portable PAM systems have improve applicability several fields, making it versatile. In this study, we laboratory-customized, two-axis, waterproof, galvanometer scanner-based (WP-GVS-HH-PAM), which provides an extended field view (14.5 × 9 mm2) wide-range The fully waterproof probe enables free movement regardless sample shape, volume rate scanning region are adjustable per experimental conditions. Results WP-GVS-HH-PAM-based phantom vivo mouse tissues (ear, iris, brain) confirm the feasibility our system as modality various biomedical applications.
منابع مشابه
A PDMS-Based 2-Axis Waterproof Scanner for Photoacoustic Microscopy
Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging tool to provide in vivo optically sensitive images in biomedical research. To achieve a small size, fast imaging speed, wide scan range, and high signal-to-noise ratios (SNRs) in a water environment, we introduce a polydimethylsiloxane (PDMS)-based 2-axis scanner for a flexible and waterproof structure. The design, theoretical b...
متن کاملHandheld photoacoustic microscopy to detect melanoma depth in vivo.
We developed handheld photoacoustic microscopy (PAM) to detect melanoma and determine tumor depth in nude mice in vivo. Compared to our previous PAM system for melanoma imaging, a new light delivery mechanism is introduced to improve light penetration. We show that melanomas with 4.1 and 3.7 mm thicknesses can be successfully detected in phantom and in in vivo experiments, respectively. With it...
متن کاملHybrid-scanning optical-resolution photoacoustic microscopy for in vivo vasculature imaging.
Recently developed optical-resolution photoacoustic microscopy (OR-PAM), which is based on the detection of optical absorption contrast, is complementary to other optical microscopy modalities such as optical confocal microscopy, optical coherence tomography, and multiphoton microscopy. A hybrid optical-mechanical scanning configuration increases the imaging speed of OR-PAM significantly, facil...
متن کاملHigh-speed and high-SNR photoacoustic microscopy based on a galvanometer mirror in non-conducting liquid
Optical-resolution photoacoustic microscopy (OR-PAM), a promising microscopic imaging technique with high ultrasound resolution and superior optical sensitivity, can provide anatomical, functional, and molecular information at scales ranging from the microvasculature to single red blood cells. In particular, real-time OR-PAM imaging with a high signal-to-noise ratio (SNR) is a prerequisite for ...
متن کاملFunctional photoacoustic microscopy of diabetic vasculature.
We used functional photoacoustic microscopy to image diabetes-induced damage to the microvasculature. To produce an animal model for Type 1 diabetes, we used streptozotocin (STZ), which is particularly toxic to the insulin-producing beta cells of the pancreas in mammals. A set number of ND4 Swiss Webster mice received intraperitoneal injections of STZ for five consecutive days at 50 mg/kg. Most...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Photonics
سال: 2021
ISSN: ['2304-6732']
DOI: https://doi.org/10.3390/photonics8080305